GAD65 Antikörper (Internal Region)

Produktnummer ABIN185388

Der Ziege Polyklonal anti-GAD65 Antikörper (ABIN185388) detektiert spezifisch GAD65 in WB, IHC und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für GAD65 Antikörper (Internal Region) (ABIN185388)

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GAD65 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-GAD65 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: GAD2 / GAD65

Sequenz: TLEDNEERMS RLSK

Spezifität: This antibody is expected to recognise both reported variants.

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-TLEDNEERMSRLSK

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 6-8 μg/mL.

Western Blot: Approx 65 kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 65.4 kDa according to NP_000809.1). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Andere Bezeichnung: GAD2

Hintergrund: GAD2, GAD65, glutamate decarboxylase 2 (pancreatic islets and brain, 65 kDa), Glutamate decarboxylase-2 (pancreas), glutamate decarboxylase 2 (pancreatic islets and brain, 65kD), RP11-420F12.2, MGC161605, MGC161607, Glutamate decarboxylase-2 (pancreas), gl

Gen-ID: 2572

NCBI Accession: NP_000809, NP_001127838