PSMD2 Antikörper (C-Term)

Produktnummer ABIN185319

Der Ziege Polyklonal anti-PSMD2 Antikörper (ABIN185319) detektiert spezifisch PSMD2 in WB, ELISA, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für PSMD2 Antikörper (C-Term) (ABIN185319)

Target: PSMD2 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 2 (PSMD2))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PSMD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-PSMD2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: TRAP2 / Proteasome subunit 26S

Sequenz: VILRKNPNYD L

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-VILRKNPNYDL, from the C Terminus of the protein sequence according to NP_002799.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 100 kDa band observed in lysates of cell lines HeLa, U251, NIH3T3 and KNRK (calculated MW of 100 kDa according to Human NP_002799.3, Mouse NP_598862.1 and Rat NP_001026809.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.<

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PSMD2

Target: PSMD2 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 2 (PSMD2))

Andere Bezeichnung: PSMD2

Hintergrund: PSMD2, TRAP2, MGC14274, S2, P97, proteasome (prosome, macropain) 26S subunit, non-ATPase, 2, 55.11 protein, TNFR-associated protein 2, 26S proteasome subunit p97, 26S proteasome regulatory subunit S2, 26S proteasome non-ATPase regulatory subunit 2, tumor

Gen-ID: 5708

NCBI Accession: NP_002799, NP_001265637, NP_001265638

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway