Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1) Antikörper

Antigen: Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1)

Synonyme: FDG, UNG3, HMUDG, MGC104370, SMUG1, C85220, 1200013B09Rik, A930006H09Rik, MGC84842, xsmug1

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185204

Produktbeschreibung

Weitere Bezeichnung: SMUG1

Gen-ID: 23583

Immunogen: PQAFLLGSIHEPA-C

Format: Affinity-purified

Beschreibung: All names and symbols: SMUG1, single-strand selective monofunctional uracil DNA glycosylase, FDG, HMUDG, UNG3. Official Symbol: SMUG1. RefSeq number(s): NP_055126. Gene ontology terms: uracil DNA N-glycosylase activity, single-stranded DNA binding activity, DNA repair

Spezifität: Human 270 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 35kDa band observed in Molt-4 lysates (predicted MW of 31kDa according to NP_055126. Recommended for use at 0.5-2µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, DNS/RNS

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185204 staining (0.5µg/ml) of Molt-4 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publikationen

Publikationen: Boorstein, Cummings, Marenstein et al.: "Definitive identification of mammalian 5-hydroxymethyluracil DNA N-glycosylase activity as SMUG1." in: The Journal of biological chemistry, Vol. 276, Issue 45, pp. 41991-7, 2001 (PubMed).