KPNA2 Antikörper (C-Term)

Produktnummer ABIN185157

Der Ziege Polyklonal anti-KPNA2 Antikörper (ABIN185157) detektiert spezifisch KPNA2 in WB, IHC, ELISA, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Ratte.

Kurzübersicht für KPNA2 Antikörper (C-Term) (ABIN185157)

Target: KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KPNA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-KPNA2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: KPNA2 / IPOA1

Sequenz: QVQDGAPGTF NF

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QVQDGAPGTFNF, from the C Terminus of the protein sequence according to NP_002257.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx 55 kDa band observed in lysates of cell lines CaCo-2, Jurkat, and KNRK, and approx. 58 kDa in lysates of cell lines MCF7 and A549 (calculated MW of 57.9 kDa according to Human NP_002257.1 and Rat NP_001257731.1). Recommended concentra

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in U2OS and A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu KPNA2

Target: KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))

Andere Bezeichnung: KPNA2

Hintergrund: KPNA2, IPOA1 QIP2, RCH1, SRP1alpha, karyopherin alpha 2 (RAG cohort 1, importin alpha 1), RAG cohort 1, importin alpha 1, importin alpha 2, importin-alpha-P1, karyopherin alpha 2, pendulin

Gen-ID: 3838, 16647, 85245

NCBI Accession: NP_002257

Pathways: M Phase, Protein targeting to Nucleus