SUMO1 Activating Enzyme Subunit 2 (UBA2) Antikörper

Antigen: SUMO1 Activating Enzyme Subunit 2 (UBA2)

Synonyme: ARX, SAE2, FLJ13058, HRIHFB2115

Klonalität: Polyklonal

Wirt: Ziege

Applikation: ELISA

Produktnummer: ABIN185083

Produktbeschreibung

Weitere Bezeichnung: SAE2 / UBA2

Gen-ID: 10054, 50995 (mouse)

Immunogen: ALSRGLPRELAEA-C

Format: Affinity-purified

Beschreibung: All names and symbols: UBA2, SAE2, SUMO-1 activating enzyme subunit 2. Official Symbol: SAE2. RefSeq number(s): NP_005490. Gene ontology terms: ubiquitin-like activating enzyme activity, ubiquitin activating enzyme activity, enzyme activator activity, catalytic activity, ubiquitin cycle

Spezifität: Human, Mouse

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments showed an approx. 35kDa band in Human Testis and Brain extracts at 1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 74KDa according to NP_005490. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Proteolyse / Ubiquitin

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).