Zinc Finger, FYVE Domain Containing 20 (ZFYVE20) Antikörper

Antigen: Zinc Finger, FYVE Domain Containing 20 (ZFYVE20)

Synonyme: ZFYVE20, zfyve20, FLJ34993, MGC126210, Rabenosyn-5, 5330426D11Rik

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185061

Produktbeschreibung

Weitere Bezeichnung: Rabenosyn 5

Gen-ID: 64145

Immunogen: C-ELKHTLAKQKGGTD

Format: Affinity-purified

Beschreibung: All names and symbols: Rabenosyn 5, FYVE-finger-containing Rab5 effector protein rabenosyn-5, zinc finger, FYVE domain containing 20, ZFYVE20. Official Symbol: ZFYVE20. RefSeq number(s): NP_071735

Spezifität: Human 784 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 110 kDa band observed in A431, Fibroblast (epidermoid carcinoma) lysates (calculated MW of 88.8 kDa according to NP_071735). Band migration is consistent with literature (PMID 11062261, 15020713). Recommended concentration: 0.3 - 1.5 µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, Chromatin-Bindungsproteine, Transkriptionsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185061 (0.3µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Nielsen, Christoforidis, Uttenweiler-Joseph et al.: "Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain." in: The Journal of cell biology, Vol. 151, Issue 3, pp. 601-12, 2000 (PubMed).