Caspase Recruitment Domain Family, Member 11 (CARD11) Antikörper

Antigen: Caspase Recruitment Domain Family, Member 11 (CARD11)

Synonyme: BIMP3, CARMA1, MGC133069, 0610008L17Rik, 2410011D02Rik, CARD11, MGC165904, LOC100232755

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN184957

Produktbeschreibung

Weitere Bezeichnung: CARD11

Gen-ID: 84433, 108723 (mouse)

Immunogen: C-QRKTIWVDEDQL

Format: Affinity-purified

Beschreibung: All names and symbols: CARD11, caspase recruitment domain family, member 11, BIMP3, CARMA1, MGC133069, card-maguk protein 1, bcl10-interacting maguk protein 3. Official Symbol: CARD11. RefSeq number(s): NP_115791.2

Spezifität: Human, Mouse

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 150+65kDa bands observed in lysates of cell line Jurkat (calculated MW of 133kDa according to NP_115791.2). A similar pattern was observed in HEK293 transiently expressing CARD11. These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Krebs, Apoptose/Nekrose, Adaptive Immunität

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN184957 staining (1µg/ml) of human Jurkat cells (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HEK293 overexpressing CARD11 (RC222740) and probed with ABIN184957 (mock transfection in first lane), tested by Origene.

Publikationen

Publikationen: Bertin, Wang, Guo et al.: "CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B." in: The Journal of biological chemistry, Vol. 276, Issue 15, pp. 11877-82, 2001 (PubMed).