NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80) Antikörper

Antigen: NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80)

Synonyme: HEC, HEC1, TID3, KNTC2, hsNDC80, Kntc2, AV139730, 2610020P18Rik, CG9938, DmelCG9938, dmNdc80, Hec1, ndc80, wu:fb52e02, zgc:111801, NDC80

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN184821

Produktbeschreibung

Weitere Bezeichnung: HEC1

Gen-ID: 10403

Immunogen: C-YEKKATLIKSSEE

Format: Affinity-purified

Beschreibung: All names and symbols: HEC1, HEC, highly expressed in cancer, rich in leucine heptad repeats, HEC-1. Official Symbol: KNTC2. RefSeq number(s): NP_006092. Gene ontology terms: mitotic chromosome segregation, mitosis, centromere, nucleus

Spezifität: Human 642 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 75-80kDa band observed in Hela lysate (predicted MW of 74kDa according to NP_006092). Recommended for use at 0.5-2µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Krebs, Zellzyklus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN184821 staining (0.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publikationen

Publikationen: Chen, Riley, Chen et al.: "HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 6049-56, 1997 (PubMed).