PTGS2 Antikörper (C-Term)

Produktnummer ABIN184672

Dieser Ziege Polyklonal Antikörper detektiert spezifisch PTGS2 in WB, ELISA, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für PTGS2 Antikörper (C-Term) (ABIN184672)

Target: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PTGS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-PTGS2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: COX2 / PTGS2

Sequenz: NPTVLLKERS TEL

Kreuzreaktivität: Rind (Kuh), Hund, Human, Schwein

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NPTVLLKERSTEL, from the C Terminus of the protein sequence according to NP_000954.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 75-80 kDa band observed in lysates of cell lines A549 and Daudi (predicted MW of 69 kDa according to NP_000954.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3 μg/mL. Primary incubatio

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and vesicles of HepG2 and NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommende

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PTGS2

Target: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Andere Bezeichnung: PTGS2

Hintergrund: PTGS2, COX2, prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), COX-2, PHS-2, PGG/HS, PGHS-2, hCox-2, prostaglandin G/H synthase and cyclooxygenase, GRIPGHS, cyclooxygenase 2b

Gen-ID: 5743

NCBI Accession: NP_000954

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation