CUB Domain Containing Protein 1 (CDCP1) (C-Term) Antikörper

Antigen: CUB Domain Containing Protein 1 (CDCP1)

Synonyme: CD318, TRASK, SIMA135

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN184664

Produktbeschreibung

Weitere Bezeichnung: CDCP1 (isoform 1: C term)

Gen-ID: 64866

Immunogen: C-PLLNTQEPMEPAE

Format: Affinity-purified

Beschreibung: All names and symbols: CDCP1, CUB domain containing protein 1 , HGNC:24357, CD318, SIMA135, TRASK , CUB domain-containing protein 1, transmembrane and associated with src kinases. Official Symbol: CDCP1. RefSeq number(s): NP_073753.3

Spezifität: This antibody is expected to recognize isoform 1 (NP_073753.3) only. Human

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 90kDa band observed in Human Colon lysates (calculated MW of 92.3kDa according to NP_073753.3). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN184664 (1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing CDCP1 (RC220633) and probed with ABIN184664 (mock transfection in first lane), tested by Origene.

Publikationen

Publikationen: Hooper, Zijlstra, Aimes et al.: "Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen." in: Oncogene, Vol. 22, Issue 12, pp. 1783-94, 2003 (PubMed).