Band 3/AE1 Antikörper (N-Term)

Produktnummer ABIN182882

Der Kaninchen Polyklonal anti-Band 3/AE1 Antikörper wird verwendet zum Nachweis von Band 3/AE1 in Proben von Human, Kaninchen und Pferd. Er wurde validiert für WB.

Kurzübersicht für Band 3/AE1 Antikörper (N-Term) (ABIN182882)

Target: Band 3/AE1 (SLC4A1) (Solute Carrier Family 4, Anion Exchanger, Member 1 (erythrocyte Membrane Protein Band 3, Diego Blood Group) (SLC4A1))

Reaktivität: Human, Kaninchen, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Band 3/AE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Band 3/AE1 Antikörper

Bindungsspezifität: N-Term

Sequenz: PSQPLLPQHS SLETQLFCEQ GDGGTEGHSP SGILEKIPPD SEATLVLVGR

Homologie: Horse: 85%, Human: 100%, Rabbit: 85%

Produktmerkmale: This is a rabbit polyclonal antibody against SLC4A1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SLC4A1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 911 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu Band 3/AE1

Target: Band 3/AE1 (SLC4A1) (Solute Carrier Family 4, Anion Exchanger, Member 1 (erythrocyte Membrane Protein Band 3, Diego Blood Group) (SLC4A1))

Andere Bezeichnung: SLC4A1

Hintergrund: The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40 kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the encoded protein and is common in areas where Plasmodium falciparum malaria is endemic. One null mutation in this gene is known, resulting in very severe anemia and nephrocalcinosis.
Alias Symbols: DI, FR, SW, WD, WR, AE1, CHC, SAO, WD1, BND3, EPB3, SPH4, CD233, EMPB3, RTA1A
Protein Interaction Partner: UBC, PIN1, HECW2, CBX8, CBX5, MDC1, TRPM8, TXLNG, SBDS, TTC4, SUMO2, Mad2l2,
Protein Size: 911

Molekulargewicht: 102 kDa

Gen-ID: 6521

NCBI Accession: NM_000342, NP_000333

UniProt: P02730