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Collagen, Type I (COL1) Antikörper (Biotin)

Antigen

Collagen, Type I (COL1)
Klonalität Polyklonal
Wirt
Alternativen

Ziege
Reaktivität
Alternativen

Human
Konjugat
Alternativen Biotin
Applikation
Alternativen ELISA, Immunfluoreszenz (IF), Immunhistochemie (IHC)
Produktnummer ABIN135047
Menge 0,2 mg  (Varianten)
Preis 381,07 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 5 bis 7 Werktagen

Produktbeschreibung
Anwendungen Alternativen Varianten zurück nach oben

Weitere Bezeichnung Type I Collagen
Beschreibung Reacts with conformational determinants on human type I collagen as demonstrated by ELISA. May react with type I collagen from other species. Exhibits <10% cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Anwendungen
Alternativen Varianten zurück nach oben

Applikationshinweise WORKING DILUTIONS Immunocytochemistry/ Immunohistochemistry: Purified (1:10-1:20), Biotin (1:20-1:40). ELISA: Biotin (1:1000-1:4000). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need. *Please note that harsh chemicals or procedures involving heat may denature collagen epitopes needed for proper binding. We recommend avoiding such applications when using this antibody.
Lagerung The purified (UNLB) antibody is supplied as 0.2 mg of purified immunoglobulin in 0.5 mL (C = 0.4 mug/mL) of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.2 mg in 0.5 mL (C = 0.4 mug/mL) of PBS/NaN3. Store at 2-8C. Reagents are stable for the period shown on the label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Beschränkungen Nur für Forschungszwecke einsetzbar

Alternativen
Anwendungen Varianten zurück nach oben

Alternativen zu Antigen "Collagen, Type I (COL1)", Typ "Antikörper" finden
Wirte Kaninchen (82), Ziege (16), Maus (16), Rind (Kuh) (2), Ratte (2), Human (1)
Reaktivitäten Human (72), Ratte (Rattus) (33), Maus (29), Rind (Kuh) (27), Hund (19), Pferd (18), Schaf (16), Kaninchen (15), Huhn (9), Fisch (6), Vogel (3), Amphibien (2), Säugetiere (2), Schwein (2), Goldfisch (1), Lachs (Salmonidae) (1), Thunfisch (1)
Applikationen ELISA (87), Immunfluoreszenz (IF) (62), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (57), Immunhistochemie (Paraffinschnitte) (IHC (p)) (37), Western Blot (WB) (30), Radioimmunoassay (RIA) (28), Durchflusszytometrie (FACS) (18), Immunzytochemie (ICC) (11), Immunhistochemie (IHC) (9), Immunpräzipitation (IP) (9), Dot Blot (Dot) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Festphasen Radioimmunoassay (SP-RIA) (1)
Konjugate Biotin (10), None (5), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)