Ziege anti-Maus Immunoglobulin G (IgG) Antikörper (PE)

Antigen: Immunoglobulin G (IgG)

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Maus

Konjugat: PE

Applikation: Fluoreszenzmikroskopie (FM), Durchflusszytometrie (FACS)

Produktnummer: ABIN129745

Produktbeschreibung

Weitere Bezeichnung: Immunoglobulin G

Immunogen: Mouse IgG whole molecule

Format: Liquid (sterile filtered)

Beschreibung: Phycoerythrin Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat] Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. Label: R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons). Absorption Wavelength: 488 nm. Emission Wavelength: 575 nm. OD566nm/OD280nm: 3.0.

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Anwendungen

Applikationshinweise: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Reinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free. 0.01% (w/v) Sodium Azide.

Lagerung: Store vial at 4° C prior to opening. DO NOT FREEZE. Restore with 1.0 ml of deionized water. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date for properly stored material is six (6) months from date of opening.

Forschungsgebiet: Sekundärantikörper

Beschränkungen: Nur für Forschungszwecke einsetzbar