Cyclin E2 (CCNE2) Antikörper

Antigen: Cyclin E2 (CCNE2)

Synonyme: CYCE2, ccne2, MGC53684, MGC132360, cb165, sb:cb165, zgc:86724, CCNE2, cyce2

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129714

Produktbeschreibung

Weitere Bezeichnung: Cyclin E2

Gen-ID: AAC80527

Swiss-Prot: Q9Z238

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the carboxyl terminus of the Cyclin E2 protein.

Format: Liquid (sterile filtered)

Beschreibung: Cyclin E was first identified by its ability to rescue growth of yeast deficient in G1 Cyclins, indicating a role in G1 or G1/S transitions. Over-expression of Cyclin E has been observed in a variety of human tumors. Multiple isoforms of Cyclin E are expressed in tumors but not in normal tissues, suggesting a post-transcriptional regulation of Cyclin E. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27Kip1 and p21Cip1. The catalytic activity associated with Cyclin E2 complexes is cell cycle regulated and peaks at the G1/S transition. Unlike Cyclin E1, which is expressed in most proliferating normal and tumor cells, Cyclin E2 levels were low to undetectable in non-transformed cells and increased significantly in tumor-derived cells.

Spezifität: This affinity purified antibody is directed against mouse Cyclin E2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Cyclin E2 protein from human based on 100% homology with the immunizing sequence. Cross-reactivity with Cyclin E2 from rat is also predicted based on a 91% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:20,000 - 1:85,000 WESTERN BLOT 1:200 - 1:2,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to Cyclin E2 protein by western blotting in the appropriate cell lysate or extract.

Konzentration: 1.17 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Forschungsgebiet: Krebs, Zellzyklus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Cyclin E2 (CCNE2) antibody