ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) Antikörper

Antigen: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1)

Synonyme: CLCS, MDR1, P-GP, PGY1, ABC20, CD243, GP170, MGC163296, Abcb1, Mdr1a, ABCB1, ABCB4, CMDR1, xemdr, p-gp

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129711

Produktbeschreibung

Weitere Bezeichnung: ATP-Binding Cassette Sub-Family B Member 1 (ABCB1)

Gen-ID: NP 000918

Swiss-Prot: P08183

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 262-277 of human ABCB1 protein.

Format: Liquid (sterile filtered)

Beschreibung: ATP-Binding Cassette Sub-Family B Member 1 (ABCB1, also named P-glycoprotein) is a plasma membrane-associated multidrug transporter that utilizes the energy of ATP hydrolysis to pump toxic xenobiotics out of cells. Unique features of ABCB1 are its very broad substrate specificity and its basal ATPase activity in the absence of transport substrates. Human ABCB1 plays an important role in absorption, distribution, metabolism, excretion and toxicity of pharmacologically relevant drugs. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anti-cancer drugs. This protein also functions as a transporter across the blood-brain barrier.

Spezifität: This affinity-purified antibody is directed against human ABCB1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross reactivity with ABCB1 protein from human, monkey, dog, mouse, and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:10,000 - 1:50,000 WESTERN BLOT 1:200 - 1:2,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized Western blot using Antibodies-Online's affinity purified anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10,000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD. 1 ug 2.5 ug 5 ug 10 ug. Note: This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 140 kDa in size corresponding to ABCB1 protein by western blotting in the appropriate cell lysate or extract.

Konzentration: 1.28 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Beschränkungen: Nur für Forschungszwecke einsetzbar