Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (pT244) Antikörper

Antigen: Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27)

Synonyme: APC3, HNUC, CDC27Hs, D0S1430E, D17S978E, wu:fb54g06, wu:fc88c12, AI452358, BC023187, MGC80529, CDC27, MGC145551, NV10210

Epitope: pT244

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129656

Produktbeschreibung

Weitere Bezeichnung: CDC27 pT244

Gen-ID: NP_001247

Swiss-Prot: P30260

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 239-249 of Human CDC27. Protein Sequence: Human CDC27, 824 aa, predicted MW 91.8 kDa 1 mtvlqepvqa aiwqalnhya yrdavflaer lyaevhseea lfllatcyyr sgkaykayrl 61 lkghscttpq ckyllakccv dlsklaegeq ilsggvfnkq kshddivtef gdsacftlsl 121 lghvycktdr lakgsecyqk slslnpflws pfeslceige kpdpdqtfkf tslqnfsncl 181 pnscttqvpn hslshrqpet vltetpqdti elnrlnless nskyslntds svsyidsavi 241 spd t vplgtg tsilskqvqn kpktgrsllg gpaalspltp sfgilpletp spgdgsylqn 301 ytntppvidv pstgapskks varigqtgtk svfsqsgnsr evtpilaqtq ssgpqtsttp 361 qvlsptitsp pnalprrssr lftsdssttk enskklkmkf ppkipnrktk sktnkggitq 421 pnindsleit kldssiiseg kistitpqiq afnlqkaaaa glmsllremg kgylalcsyn 481 ckeainilsh lpshhyntgw vlcqigrayf elseymqaer ifsevrrien yrvegmeiys 541 ttlwhlqkdv alsvlskdlt dmdknspeaw caagncfslq rehdiaikff qraiqvdpny 601 ayaytllghe fvlteeldka lacfrnairv nprhynawyg lgmiyykqek fslaemhfqk 661 aldinpqssv llchigvvqh alkksekald tlnkaividp knplckfhra svlfanekyk 721 salqeleelk qivpkeslvy fligkvykkl gqthlalmnf swamdldpkg annqikeaid 781 krylpddeep itqeeqimgt desqessmtd addtqlhaae sdef

Format: Liquid (sterile filtered)

Beschreibung: Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.

Spezifität: This affinity purified antibody is directed against the phosphorylated form of human CDC27 at the pT244 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human CDC27 pT244 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human CDC27 is minimal by ELISA. The antibody does not cross-react with CDC27 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, bovine and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with CDC27 protein from mouse, rat and chicken may occur as sequence homology varies by one amino acid residue in this sequence (90% homology). Cross reactivity with CDC27 homologues from other sources has not been determined.

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:8,000 - 1:30,000 WESTERN BLOT 1:300 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized 1 2 200. Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 92 kDa in size corresponding to CDC27 by western blotting in the appropriate cell lysate or extract. Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pT244 of CDC27.

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar