Slit Homolog 2 (Drosophila) (SLIT2) Antikörper

Antigen: Slit Homolog 2 (Drosophila) (SLIT2)

Synonyme: SLIL3, Slit-2, FLJ14420, Slil3, Drad-1, KIAA4141, mKIAA4141, E030015M03Rik, E130320P19Rik, Slit, slit2-a

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129628

Produktbeschreibung

Weitere Bezeichnung: SLIT-2

Gen-ID: NP_004778

Swiss-Prot: O94813

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 484-500 of Human SLIT-2 protein.

Format: Liquid (sterile filtered)

Beschreibung: SLIT-2 is thought to act as a molecular guidance cue in cellular migration, and its function appears to be mediated by interaction with roundabout homologue receptors. SLIT-1 and SLIT-2 seem to be essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development SLIT-2 may play a role in guiding commissural axons once they reach the floor plate by modulating the response to netrin. In vitro, SLIT-2 silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. In vitro, only commissural axons that crossed the midline responded to SLIT-2. In the developing visual system this protein functions as a repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. SLIT-2 is a secreted protein predominantly expressed in fetal lung and kidney, and adult spinal cord. Weak expression is observed in adult adrenal gland, thyroid, trachea and other tissues examined. Multiple isoforms have been reported for this product.

Spezifität: This affinity purified antibody is directed against human SLIT-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-2 from Tetraodon nigroviridis , as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). The core 13 amino acids of the immunogen are also 100% homologous to SLIT-2 from mouse, rat, bovine and Xenopus laevis. Cross reactivity with SLIT-2 homologues from other sources has not been determined.

Anwendungen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~ 165 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. For western blotting block the membrane with goat serum. Do not block with non-fat dry milk. Dilutions: ELISA 1:1,500 - 1:6,000 WESTERN BLOT 1:500 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Konzentration: 1.33 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar