Sprouty Homolog 4 (Drosophila) (SPRY4) Antikörper

Antigen: Sprouty Homolog 4 (Drosophila) (SPRY4)

Synonyme: MGC154989, SPRY4, LOC100218545

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: Immunhistochemie (IHC), ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129626

Produktbeschreibung

Weitere Bezeichnung: SPROUTY-4

Gen-ID: NP_112226

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 306-322 of Human Sprouty-4 protein.

Format: Liquid (sterile filtered)

Beschreibung: Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.

Spezifität: This affinity purified antibody is directed against human Sprouty-4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity is expected with Sprouty-4 protein from bovine (94% homology), mouse (88% homology) and rat and dog (82% homology). Expect reactivity only with the SPRY4A splice variant of this protein. Cross reactivity with Sprouty-4 homologues from other sources has not been determined.

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:3,000 - 1:12,000 WESTERN BLOT 1:100 - 1:1,000 IMMUNOHISTOCHEMISTRY 1 ug/ml to 10 ug/ml OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band (maybe a doublet) ~35 kDa in size corresponding to Sprouty-4 by western blotting in the appropriate cell lysate or extract. Some non-specific banding may be observed using this antibody.

Konzentration: 0.93 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Sprouty Homolog 4 (Drosophila) (SPRY4) antibody

0.93 mg/ml (by UV absorbance at 280 nm)