SPRY4 Antikörper (AA 275-299)

Produktnummer ABIN129626

Hersteller Produkt- Nr.: 600-401-697

Der Kaninchen Polyklonal anti-SPRY4 Antikörper wird verwendet zum Nachweis von SPRY4 in Proben von Human und Maus. Er wurde validiert für WB, ELISA und IHC.

Kurzübersicht für SPRY4 Antikörper (AA 275-299) (ABIN129626)

Target: SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPRY4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-SPRY4 Antikörper

Bindungsspezifität: AA 275-299

Hersteller Produkt- Nr.: 600-401-697

Hersteller: Rockland

Verwendungszweck: Sprouty-4 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human Sprouty-4 protein.

Produktmerkmale: Synonyms: Rabbit anti-Sprouty4 antibody, Rabbit anti-Sprouty-4 antibody, Sprouty 4, SPRY4, SPRY 4, Spry-4, Protein sprouty homolog 4

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 275-299 of Human Sprouty-4 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1 μg/mL to 10 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band (maybe a doublet) ~35 kDa in size corresponding to Sprouty-4 by western blotting in the appropriate cell lysate or extract.  Some non-specific banding may be observed using this antibody.

Western Blot Dilution: 1:100 - 1:1,000

ELISA Dilution: 1:3,000 - 1:12,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu SPRY4

Target: SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))

Andere Bezeichnung: SPRY4

Hintergrund: Background: Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.  SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.

Gen-ID: 81848

NCBI Accession: NP_001120968

UniProt: Q9C004