Aurora Kinase B (AURKB) (pT232) Antikörper

Antigen: Aurora Kinase B (AURKB)

Synonyme: AIK2, AIM1, ARK2, AurB, IPL1, STK5, AIM-1, STK12, aurkb-sv1, aurkb-sv2, AIE1, AIK3, Stk13, Aik2, Aim1, Ark2, Stk5, AIRK2, STK-1, Stk12, AL022959, DmAIRK2, DmAurB, DmAuroraB, IAL, IAL1, aurora B, DmelCG6620, CG6620, AURKB, MGC142782, aik2, aim1, ark2, ipl1, stk5, aim-1, stk12, Aurora-B, fa09g06, MGC85918, CHUNP6896, wu:fa09g06, aurkb

Epitope: pT232

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129612

Produktbeschreibung

Weitere Bezeichnung: Aurora B pT232

Gen-ID: NP_004208.

Swiss-Prot: Q96GD4

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 223-234 of Human Aurora Kinase B protein. Protein Sequence: Human Aurora B Kinase, 344 aa, predicted MW 39.3kDa 1 maqkensypw pygrqtapsg lstlpqrvlr kepvtpsalv lmsrsnvqpt aapgqkvmen 61 ssgtpdiltr hftiddfeig rplgkgkfgn vylarekksh fivalkvlfk sqiekegveh 121 qlrreieiqa hlhhpnilrl ynyfydrrri ylileyaprg elykelqksc tfdeqrtati 181 meeladalmy chgkkvihrd ikpenlllgl kgelkiadfg wsvhapslrr k t mcgtldyl 241 ppemiegrmh nekvdlwcig vlcyellvgn ppfesashne tyrrivkvdl kfpasvptga 301 qdliskllrh npserlplaq vsahpwvran srrvlppsal qsva

Format: Liquid (sterile filtered)

Beschreibung: Aurora Kinase B (also known as Aurora and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G 2 /M phase and expression is up-regulated in cancer cells during M phase.

Spezifität: This affinity purified antibody is directed against the phosphorylated form of human Aurora Kinase B at the pT232 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Aurora Kinase B pT232 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A. However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross reactivity with Aurora Kinase B from other sources is not known.

Synonyme: AIK2, AIM1, ARK2, AurB, IPL1, STK5, AIM-1, STK12, aurkb-sv1, aurkb-sv2, AIE1, AIK3, Stk13, Aik2, Aim1, Ark2, Stk5, AIRK2, STK-1, Stk12, AL022959, DmAIRK2, DmAurB, DmAuroraB, IAL, IAL1, aurora B, DmelCG6620, CG6620, AURKB, MGC142782, aik2, aim1, ark2, ipl1, stk5, aim-1, stk12, Aurora-B, fa09g06, MGC85918, CHUNP6896, wu:fa09g06, aurkb

Anwendungen

Applikationshinweise: The affinity purified antibody has been tested for use in ELISA any by western blot. Expect a band app. 39kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract. HeLa cell lysate can be used as a positive controle.

Konzentration: 1.21 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Mackay, Makise, Ullman: "Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint." in: The Journal of cell biology, 2010 (PubMed).