Phosphoserine Antikörper

Antigen: Phosphoserine

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129572

Produktbeschreibung

Weitere Bezeichnung: Phosphoserine (pS) (Free)

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphoserine conjugated KLH.

Format: Liquid (sterile filtered)

Spezifität: This product was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated serine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG. Reactivity is specific for phosphoserine and minimal cross-reactivity is observed against phosphotyrosine or phosphothreonine.

Anwendungen

Applikationshinweise: This affinity purified antibody is suitable for use in ELISA, western blotting and immunoprecipitation assays. The antibody reacts specifically with phosphoserine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphothreonine residues. The antibody reacts with free phosphoserine, phosphoserine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphoserine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Konzentration: 1.0 mg/ml

Buffer: Stabilizer: 50% (v/v) Glycerol. Preservative: None. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Aminosäuren, Proteinmodifikationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

antibodies-online's Affinity Purified antiphosphoserine (pS) antibody is shown to detect phosphorylated proteins present in UV-treated mouse spleen cell lysates (top panel).