DNA Cross-Link Repair 1C (DCLRE1C) Antikörper

Antigen: DNA Cross-Link Repair 1C (DCLRE1C)

Synonyme: SCIDA, SNM1C, A-SCID, RS-SCID, DCLREC1C, FLJ11360, FLJ36438, Art, Snm1l, AI661365, MGC123409, 9930121L06Rik, artemis, nuclease, DCLRE1C, DKFZp459G244

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human, Maus, Ratte (Rattus), Schaf

Applikation: Immunhistochemie (IHC), ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129558

Produktbeschreibung

Weitere Bezeichnung: ARTEMIS (DCLRE1C DNA Cross-Llink Repair 1C)

Gen-ID: NP_071932

Swiss-Prot: Q96SD1

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizationswith a synthetic peptide corresponding aa 482-495 of Human ARTEMIS (DCLRE1C DNA cross-link repair 1C). Protein Sequence: Human Artemis protein, 577 aa, predicted MW 65.4 kDa 1 mkhqerflfq gnngtvlytg dfrlaqgeaa rmellhsggr vkdiqsvyld ttfcdrrfyq 61 ipsreeclsg vlelvrswit rspyhvvwln ckaaygyeyl ftnlseelgv qvhvnkldmf 121 rnmpeilhhl ttdrntqiha crhpkaeeyf qwsklpcgit srnriplhii sikpstmwfg 181 ersrktnviv rtgessyrac fsfhssysei kdflsylcpv naypnvipvg ttmdkvveil 241 kplcrssqst epkykplgkl krartvhrds eeeddylfdd plpiplrhkv pypetfhpev 301 fsmtavsekq peklrqtpgc craecmqssr ftnfvdcees nseseeevgi paslqgdlgs 361 vlhlqkadgd vpqwevffkr ndeitdesle nfpsstvagg sqspklfsds dgesthissq 421 nssqsthite qgsqgwdsqs dtvllssqer nsgditsldk adyrptiken ipaslmeqnv 481 icpkdtysdl ksrdkdvtiv pstgepttls sethipeeks llnlstnads qsssdfevps 541 tpeaelpkre hlqylyekla tgesiavkkr kcslldt

Format: Liquid (sterile filtered)

Beschreibung: Artemis has a role in T and Blymphocyte immunodeficiency and in predisposition tolymphoma through the NHEJ pathway of DNA repair. DNA-PKcs regulates Artemis by both phosphorylation and complexformation to permit enzymatic activities that are critical for thehairpin-opening step of V(D)J recombination and for the 5' and3' overhang processing in non-homologous DNA end joining.

Spezifität: This is an affinity purifiedantibody produced by immunoaffinity chromatographyusing the immunizing peptide after immobilization to asolid phase. This antibody detects a band of theappropriate size in a number of different cell lines.However, it does not detect a band in cell lines that do not express artemis, such as the cell line CJ (see figure) andin an hTERT line (not shown).

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:10,000 - 1:20,000 WESTERN BLOT 1:500 - 1:2,000 IMMUNOHISTOCHEMISTRY 1:1,000 OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and westernblotting. Reactivity in other immunoassays is unknown. This antibody detects a band of approximately 90 kDa (predictedmolecular weight: 78 kDa).

Konzentration: 1.2 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunoblotting. antibodies-online's Goat-anti-Artemis was used at a 1:500 dilution to detect Artemis is various cell lysates by Western blot. CJ is an Artemis deficient cell line and so no band is visible.

Immunohistochemistry. antibodies-online's Affinity Purified anti-Artemis antibody was used at a 1:1000 dilution to detect Artemis by immunohistochemistry in human spleen. Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.