SMT3 Suppressor of Mif Two 3 Homolog 3 (S. Cerevisiae) (SUMO3) Antikörper

Antigen: SMT3 Suppressor of Mif Two 3 Homolog 3 (S. Cerevisiae) (SUMO3)

Synonyme: SMT3A, SMT3H1, SUMO-3, Smt3h1, AA409334, AW121497, AW536077, D10Ertd345e, 2810014B19Rik, sumo3, sumo2, fa94a11, zgc:65847, zgc:85643, im:7141764, wu:fa94a11, wu:fb55d09, fc66f10, fl11h12, zgc:86902, wu:fc66f10, wu:fl11h12, SUMO3, ATSUMO3, MCO15.12, MCO15_12, SMALL UBIQUITIN-LIKE MODIFIER 3, SUM3, SUMO 3

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus, Human, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129521

Produktbeschreibung

Weitere Bezeichnung: SUMO-3

Gen-ID: NP_008867

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO-3protein. Protein Sequence: Human SUMO-3, 103 aa, predicted MW 11.6 kDa 1 mseekpkegv ktendhinlk vagqdgsvvq fkikrhtpls klmkaycerq glsmrqirfr 61 fdgqpinetd tpaqlemede dtidvfqqqt ggvpesslag hsf

Format: Liquid (sterile filtered)

Beschreibung: Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. But, incontrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability ormodulate their subcellular compartmentalization. Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs)function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12.Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins containdomains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to otherproteins. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localizationand stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to human.Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates:SUMO-1 and the close homologues SUMO-2 and SUMO-3. SUMO has been called SMT3 (yeast), sentrin, PIC1, GMP1 and UBL1. SUMOhas been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, I kappa B-alpha , Androgen receptor (AR), GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast Septins, and viral CMV-IE1/2, EBV-BZLF1, HPV/BPV-E1. These bindings implicate SUMO in the stabilization of the target proteins and/ortheir localization to subcellular complexes. SUMO has an apparent molecular weight of ~12kDa and human SUMO-1 (a 101 amino acidpolypeptide) shares 50% sequence identity with SUMO-2 and SUMO-3 and with yeast SMT3. SUMO and ubiquitin only show about 18%homology, but both possess a common three-dimensional structure characterized by a tightly packed globular fold with beta -sheets wrapped around an alpha -helix.

Spezifität: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process whichincludes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer statedabove. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Synonyme: SMT3A, SMT3H1, SUMO-3, Smt3h1, AA409334, AW121497, AW536077, D10Ertd345e, 2810014B19Rik, sumo3, sumo2, fa94a11, zgc:65847, zgc:85643, im:7141764, wu:fa94a11, wu:fb55d09, fc66f10, fl11h12, zgc:86902, wu:fc66f10, wu:fl11h12, SUMO3, ATSUMO3, MCO15.12, MCO15_12, SMALL UBIQUITIN-LIKE MODIFIER 3, SUM3, SUMO 3

Anwendungen

Applikationshinweise: This purified polyclonal antibody reacts with human SUMO-3 by western blot and ELISA. Although not tested,this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions mayrecognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions.Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 11.6 kDa in size corresponding tohuman SUMO-3 by western blotting in the appropriate cell lysate or extract.

Konzentration: 5.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at 4° C prior to restoration. Restore with 0.1 ml of deionized water (or equivalent). For extendedstorage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completelyclear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior toimmediate use. Expiration date is one (1) year from date of restoration.

Forschungsgebiet: Signaltransduktion, Proteasen, DNS/RNS

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis is shown using antibodies-online's Affinity Purified anti-Human SUMO-3 antibody to detect GFP-SUMO fusion proteins (arrowheads). Panel A. Increasing concentrations of human GFP-SUMO-3 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4