Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) Antikörper

Antigen: Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1)

Synonyme: BUB1A, BUB1L, hBUB1, BUB1, MGC148432, bub1a, bub1l, hbub1, xbub1, MGC145526

Klonalität: Polyklonal

Wirt: Maus

Reaktivität: Human

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129514

Produktbeschreibung

Weitere Bezeichnung: BUB1

Gen-ID: NP_004327

Swiss-Prot: O43683

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinantprotein corresponding to amino acid residues 281-419 of human BUB1 protein. Hybridoma: Produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.

Format: Liquid (sterile filtered)

Isotyp: IgG1  (Passende Sekundärantikörper)

Beschreibung: BUB1 (also called Mitotic checkpoint serine/threonine-protein kinase BUB1and BUB1A) is a kinase involved inspindle checkpoint function. The kinase functions in part by phosphorylating BUB3, a member of the miotic checkpointcomplex, and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and severalforms of cancer. BUB1 is autophosphorylated when cells enter mitosis. This protein is localized to the nucleus in interphasecells. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. BUB1 is highlyexpressed in testis and thymus, less in colon, spleen, lung and small intestine. Expression is associated with cells/tissues witha high mitotic index.

Spezifität: This Protein A purified antibody is directed against human BUB1 protein. The product was purifiedfrom tissue culture supernatant by chromatography. This antibody reacts with BUB1 from human cells. No reactivity is seenfor homologues from Xenopus or Kangaroo Rat. Reactivity against homologues from other sources is not known.

Anwendungen

Applikationshinweise: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescencestaining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should beoptimized by the end user. Expect a predominant band at ~ 160 kDa corresponding to full-length protein by western blotting inthe appropriate cell lysate or extract. The use of HeLa whole cell lysates prepared using a RIPA buffer is recommended as apositive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8 OR100% methanol at -20

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date ofopening.

Beschränkungen: Nur für Forschungszwecke einsetzbar