HLA-DR-gamma (CD74) Antikörper

Antigen: HLA-DR-gamma (CD74)

Synonyme: DHLAG, HLADG, Ia-GAMMA, Ii, CLIP

Klonalität: Monoklonal (MEM-12)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN125741

Produktbeschreibung

Weitere Bezeichnung: HLA-DR

Immunogen: thymocyte membrane

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: MEM-12

Spezifität: The antibody MEM-12 recognizes common epitope on human HLA-DR which is dependenton the association of alpha and beta chains. DR is the isotype of human MHC Class IImolecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes,monocytes, macrophages).

Anwendungen

Applikationshinweise: Flow Cytometry - Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Immunoprecipitation - Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. - Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing SDS-PAGE sample buffer. Do not heat/boil. Application note: Non-reducing conditions.

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Haupthistokompatibilitätskomplex (MHC), CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Flow Cytometry analysis

Publikationen

Publikationen: Simpson, McFarlin, McSporran et al.: "Toll-like receptor expression on classic and pro-inflammatory blood monocytes after acute exercise in humans." in: Brain, behavior, and immunity, Vol. 23, Issue 2, pp. 232-9, 2009 (PubMed).