CD46 Antikörper
| Antigen |
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| Klonalität |
Monoklonal (MEM-258) |
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Wirt
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Reaktivität
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Applikation
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 Alternativen Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB)
Durchflusszytometrie (FACS) (50), Western Blot (WB) (35), Immunpräzipitation (IP) (27), Immunhistochemie (Paraffinschnitte) (IHC (p)) (5), Funktionale Studien (Func) (4), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (3), Immunhistochemie (IHC) (3), ELISA (1), ELISA (Detektionsantikörper) (1), Fluoreszenzmikroskopie (FM) (1), Immunzytochemie (ICC) (1)
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| Produktnummer |
ABIN125715 |
| Menge |
0.1 mg (1 mg/ml) (Varianten) |
| Preis |
190,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 3 bis 5 Werktagen |
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Immunogen
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HPB-ALL human T cell line
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Format
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Azide Free
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Isotyp
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IgG1 (Passende Sekundärantikörper)
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Klon
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MEM-258
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Beschreibung
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CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembraneprotein that binds and inactivates C3b and C4b complement fragments, regulates Tcell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing(CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor forseveral human pathogens (both bacteria and viruses), and its ligation alteres T lymphocytepolarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function.CD46 is a protector of placental tissue and is also expressed on the inner acrosomalmembrane of spermatozoa.
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Spezifität
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The antibody MEM-258 recognizes an epitope on SCR4 (the membrane-proximal SCR)domain of CD46 (Membrane cofactor protein). CD46 is 56-66 kDa dimeric transmembraneprotein expressed on T and B lymphocytes, platelets, monocytes, granulocytes, endothelialcells, epithelial cells and fibroblast, it is negative on erythrocytes.
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Applikationshinweise
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Western Blotting. Application note: Non-reducing conditions.
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Konzentration
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1 mg/ml
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Reinheit
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> 95% (by SDS-PAGE)
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Reinigung
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Purified from ascites by protein-A affinity chromatography.
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Buffer
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Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.
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Lagerung
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Forschungsgebiet
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Immunologie, Angeborene Immunität, CD Antigene, Oberflächenrezeptoren der Immunzellen
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "CD46", Typ "Antikörper" finden
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Wirte
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Maus (59), Kaninchen (2)
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Reaktivitäten
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Human (55), Rind (Kuh) (14), Schwein (5)
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Applikationen
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Durchflusszytometrie (FACS) (50), Western Blot (WB) (35), Immunpräzipitation (IP) (27), Immunhistochemie (Paraffinschnitte) (IHC (p)) (5), Funktionale Studien (Func) (4), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (3), Immunhistochemie (IHC) (3), ELISA (1), ELISA (Detektionsantikörper) (1), Fluoreszenzmikroskopie (FM) (1), Immunzytochemie (ICC) (1)
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Konjugate
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FITC (12), PE (5), RPE (3), APC (2), Biotin (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1), Pacific Blue (1)
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