T-Cell Receptor (TCR), gamma/delta- Antikörper (Biotin)

Antigen: T-Cell Receptor (TCR), gamma/delta-

Klonalität: Monoklonal (V65)

Wirt: Maus

Reaktivität: Ratte (Rattus)

Konjugat: Biotin

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS)

Produktnummer: ABIN120792

Produktbeschreibung

Weitere Bezeichnung: T Cell Receptor (TCR) gamma/delta

Immunogen: Rat T Blasts. Donor: BALB/c spleen. Fusion Partner: X63-Ag 8.653

Isotyp: IgG1

Klon: V65

Spezifität: This monoclonal antibody detects a T cell-specific heterodimeric 48 and 50 kD cell surface protein that is expressed on greater than 90% of CD3+ abTCR- rat peripheral T lymphocytes, and identifies a dense network of dendritic cells in the epidermis as g/d T cells. Immobilized this antibody induces a strong proliferative response in gd T cell cultures supplemented with either IL-2 or IL-4.

Anwendungen

Applikationshinweise: Flow cytometry. Immunohistochemistry on frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.1 mg/ml

Buffer: PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Forschungsgebiet: Immunologie, Rezeptoren, Signaltransduktion

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Tissue Distribution by Flow Cytometry Analysis: Rat Strain: Fischer Cell Concentration : 1x106 cells per test Antibody Concentration Used: 0.2 ug/106 cells Isotypic Control: Biotin Mouse IgG1 Cell Source (Percentage of cells stained above control): Thymus (3.6%) Splenic T Cells* (5.3%) *(T cells isolated with CL102 - Cedarlane's Rat T Cell Recovery Column Kit)