Glycophorin A (MNS Blood Group) (GYPA) Antikörper

Antigen: Glycophorin A (MNS Blood Group) (GYPA)

Synonyme: MN, GPA, MNS, GPSAT, CD235a, GPErik, HGpMiV, HGpMiX, GpMiIII, HGpMiXI, HGpMiIII, HGpSta(C), AI853584, GYPA, gpa

Klonalität: Monoklonal (HIR2)

Wirt: Maus

Reaktivität: Human

Applikation: Aggregation (AGG), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS)

Produktnummer: ABIN120751

Produktbeschreibung

Weitere Bezeichnung: CD235a / GYPA

Swiss-Prot: P02724

Immunogen: Synthetic peptide (Human, N-terminal)

Isotyp: IgG2b  (Passende Sekundärantikörper)

Klon: HIR2

Beschreibung: CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin. Alternate Names: GYPA, GPA, CD235A, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, Glycophorin-A.

Spezifität: TThe antibody HIR2 recognizes N-terminal portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.

Anwendungen

Applikationshinweise: Flow cytometry: This HIR2 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes and cell agglutination assay and can be used at approximately 0.1 ug per million cells. Immunohistochemistry (frozen sections). Agglutination: Clone HIR2 agglutinates untreated RBCs but it failes to agglutinate papain- treated cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: PBS with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar