Cytokeratin 7 Antikörper

Produktnummer ABIN120627

Der Maus Monoklonal anti-Cytokeratin 7 Antikörper (Klon C-46) (ABIN120627) detektiert spezifisch Cytokeratin 7 in WB, IF, IHC (p), IP und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Rind (Kuh).

Kurzübersicht für Cytokeratin 7 Antikörper (ABIN120627)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reaktivität: Human, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: C-46

Produktdetails anti-Cytokeratin 7 Antikörper

Spezifität: The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa).

Kreuzreaktivität (Details): Species reactivity (tested):Human, Porcine, Bovine.
Also reacts to: KRT17

Produktmerkmale: Synonyms: KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin, Keratin type II cytoskeletal 7

Aufreinigung: Protein A-affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Immunogen: Cytoskeleton preparation of HeLa human cervix carcinoma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation. Immunocytochemistry. Western Blot: 2-3 mg/mL, 60 min in RT, Boil for 3 min, Resuspend dry pelet in solubilizelysis buffer (with carbamide and detergent NP-40), Mix lysate with reducing SDS-PAGE (+merkaptoethanol) sample buffer, Reducing conditions. Positive control: HeLa human cell line. Immunohistochemistry on paraffin sections: 20 mg/mL, 0,1 % pepsin, 30 min, 37C, 4Covernight, chromogen DAB. Positive tissue: pancreas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Andere Bezeichnung: Cytokeratin 7

Hintergrund: Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Synonyms: CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin

Gen-ID: 3855

UniProt: P08729