LAG3 Antikörper

Produktnummer ABIN120323

Der Ratte Monoklonal anti-LAG3 Antikörper wird verwendet zum Nachweis von LAG3 in Proben von Maus. Er wurde validiert für FACS.

Kurzübersicht für LAG3 Antikörper (ABIN120323)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser LAG3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Klon: C9B7W

Produktdetails anti-LAG3 Antikörper

Spezifität: This antibody recognises lymphocyte activation gene-3 (LAG-3)that is also referred to as CD223. Clone C9B7W recognises an epitope within the D2 domain of CD223. The antibody is reported to block the in vitro function of murine LAG-3 but studies suggest that the antibody does not block binding of LAG-3 to MHC class II. We recommend the use of SM2268LE for this purpose.

Aufreinigung: Affinity chromatography on Protein G

Immunogen: Murine CD223 Ig fusion protein. Cells from immunised Lewis rats were fused with cells of the Sp/20 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 1/50 - 1/200, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Andere Bezeichnung: CD223 / LAG3

Hintergrund: CD223 is a 70kD activation-induced cell surface molecule. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion.Synonyms: FDC, LAG-3, Lymphocyte activation gene 3 protein

Gen-ID: 16768

NCBI Accession: NP_032505

UniProt: Q61790

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints