MADCAM1 Antikörper

Produktnummer ABIN120242

Der Maus Monoklonal anti-MADCAM1 Antikörper wird verwendet zum Nachweis von MADCAM1 in Proben von Human. Er wurde validiert für WB, FACS, IHC (p) und EIA.

Kurzübersicht für MADCAM1 Antikörper (ABIN120242)

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MADCAM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: 17F5

Produktdetails anti-MADCAM1 Antikörper

Spezifität: This antibody recognizes Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM-1). Clone 17F5 recognises the Mucin domain of MAdCAM-1. This antibody does not block the function of MAdCAM-1.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Recombinant soluble MAdCAM-1-fc fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 1/10- 1/100, use 10 μL of the suggested working dilution to label 106 cells in 100 μL. Immunohistochemistry on paraffin embedded sections: 1/50 - 1/100, This product does not require protein digestion prior to staining, This product does not require HIER prior to staining. ELISA. Western Blot: under reducing conditions, This antibody detects a band of approximately 60 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu MADCAM1

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Andere Bezeichnung: MAdCAM1

Hintergrund: Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM-1) is a 60kD transmembrane protein that belongs to the immunoglobulin superfamily. MAdCAM-1 is strongly expressed on mucosal lymphoid tissue and high endothelial venules of Peyers patches. Human MAdCAM-1 interacts with the beta 7 integrin LPAM-1 (alpha4beta7), CD62L, and VLA-4 (alpha4beta1) and is involved in lymphocyte trafficking.Synonyms: MAdCAM-1, Mucosal addressin cell adhesion molecule 1, hMAdCAM-1

Gen-ID: 8174

NCBI Accession: NP_570116

UniProt: Q13477