His Tag Antikörper

Produktnummer ABIN119354

Der Maus Monoklonal anti-His Tag Antikörper wird verwendet zum Nachweis von His Tag. Er wurde validiert für WB, IP, IHC (fro) und EIA.

Kurzübersicht für His Tag Antikörper (ABIN119354)

Target: His Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: AD1-1-10

Produktdetails anti-His Tag Antikörper

Sequenz: HHHHHH

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: PAX6 transcription factor linked to Histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunhistology on Frozen Sections. ELISA. Western Blot. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as a preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu His Tag

Target: His Tag

Andere Bezeichnung: 6xHistidine Epitope Tag

Substanzklasse: Tag

Hintergrund: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.