His Tag Antikörper (HRP)

Produktnummer ABIN119352

Der HRP-konjugierte Maus Monoklonal anti-His Tag Antikörper (Klon AD1-1-10) (ABIN119352) detektiert spezifisch His Tag in WB und EIA.

Kurzübersicht für His Tag Antikörper (HRP) (ABIN119352)

Target: His Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: AD1-1-10

Produktdetails anti-His Tag Antikörper (HRP)

Sequenz: HHHHHH

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: PAX6 transcription factor linked to histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 1/20 - 1/1000. Western Blot: 1/1000 - 1/5000, In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.01 % Thiomersal as preservative and a HRP stabilizer

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Details zu His Tag

Target: His Tag

Andere Bezeichnung: 6xHistidine Epitope Tag

Substanzklasse: Tag

Hintergrund: 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.