Fibroblasts, Epithelial Cells Antikörper

Produktnummer ABIN118888

Dieser Maus Monoklonal Antikörper detektiert spezifisch Fibroblasts, Epithelial Cells in FACS, IP und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human.Es wurde in 1 Publikation zitiert.

Kurzübersicht für Fibroblasts, Epithelial Cells Antikörper (ABIN118888)

Target: Fibroblasts, Epithelial Cells

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: D7-FIB

Produktdetails

Spezifität: SM1214P recognises a 112 kDa molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS but resistant to trypsin, tunicamycin and collagenase. In immunohistochemical studies the antibody has also been found to bind to epithelium, myoepithelium, smooth muscle cells and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (Van Osch et al., 2001).

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Human foreskin Fibroblasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: use 10 μL of a 1:50 - 1:200 dilution to label 10^6 cells in 100 μL. This product is routinely tested in flow cytometry on the KG1 cell line. Immunohistochemistry on frozen tissues or cells (1:100): use ice-cold methanol for 5 min as fixative. Does not work on paraffin sections. Can also be used for immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen

Nazareth, Broderick, Simpson-Abelson, Kelleher, Yokota, Bankert: "Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 9, pp. 5552-62, (2007) (PubMed).

Antigendetails

Target: Fibroblasts, Epithelial Cells

Andere Bezeichnung: Fibroblasts / Epithelial Cells

Hintergrund: A fibroblast is a connective-tissue cell of mesenchymal (somewhat undifferentiated) origin that secretes proteins from which the extracellular fibrillar matrix of connective tissue forms. Epithelial cells are cells that cover the surface of the body and line its cavities.Synonyms: Fibroblast Marker, Fibroblasten