Interleukin 4 (IL4) Antikörper

Antigen: Interleukin 4 (IL4)

Synonyme: BSF1, IL-4, BCGF1, BCGF-1, MGC79402, Il-4, IL4, Il4e12, IL-2, BSF-1

Klonalität: Monoklonal (BVD4-1D11)

Wirt: Ratte

Reaktivität: Maus

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), ELISA (Fangantikörper), Durchflusszytometrie (FACS), Western Blot (WB)

Produktnummer: ABIN118404

Produktbeschreibung

Weitere Bezeichnung: Interleukin-4 / IL4

Swiss-Prot: P07750

Immunogen: Recombinant Mouse IL-4. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: BVD4-1D11

Beschreibung: Interleukin-4 is a potent lymphoid growth factor, which stimulates the growth and survival of certain B and T cells.

Spezifität: This antibody is specific for Interleukin-4 (IL-4). Clone BVD4-1D11 is reported to neutralise the effects of IL-4. We recommend the use of ABIN118403 for functional studies.

Anwendungen

Applikationshinweise: Flow Cytometry: 1/10 - 1/50, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul, Membrane permeabilisation is required. Immunohistochemistry on frozen sections. ELISA: 1ug/ml - 5ug/ml. Western Blot: 1ug/ml - 5ug/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: PBS, pH7.4 containing 0.09% Sodium Azide

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of mouse IL-4 recombinant protein probed with RAT ANTI MOUSE INTERLEUKIN-4 (ABIN118404) followed by F(ab')2 RABBIT ANTI RAT IgG:HRP

Publikationen

Publikationen: Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).

Sander, Hoeiduen, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).

Hochrein, OKeeffe, Luft et al.: "Interleukin (IL)-4 is a major regulatory cytokine governing bioactive IL-12 production by mouse and human dendritic cells." in: The Journal of experimental medicine, Vol. 192, Issue 6, pp. 823-33, 2000 (PubMed).