Urease Antikörper
Kurzübersicht für Urease Antikörper (ABIN116845)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
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Aufreinigung
- Prepared from monospecific antiserum by a Delipidation and Defibrination
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Immunogen
- Urease [Jack Bean].
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Applikationshinweise
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Diutions: This product has been assayed against 1.0 μg of Urease [JackBean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 1,000 to1: 3,000 of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Rekonstitution
- Restore with 2.0 mL of deionized water (or equivalent).
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Konzentration
- 90.0 mg/mL (by Refractometry)
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Buffer
- 0.01 M Sodium Phosphate, 0.11 M Sodium Chloride, pH 7.4, 0.01 % Sodium Azide, None
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Avoid repeated freezing and thawing.
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Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Urease (URE)
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Andere Bezeichnung
- Urease
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Hintergrund
- Urease is a protein that is commonly secreted from the bacterium H. Pylori and is integral to the immune response within the gastric system. Urease carries out a key enzymatic reaction in converting urea into free ammonia and carbonic acid. Urease is commonly derived from the Jack Bean plant in scientific research as the amino acid sequences are highly conserved between species and it is an abundant source of the protein. Anti-Urease Antibody is ideal for investigators in Immunology, Enzymology, and Cell Biology research.Synonyms: Urea amidohydrolase
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Gen-ID
- 3823
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UniProt
- P07374
Target
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