CXCL1 Antikörper

Produktnummer ABIN115941

Der Kaninchen Polyklonal anti-CXCL1 Antikörper (ABIN115941) detektiert spezifisch CXCL1 in WB, EIA und Func. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für CXCL1 Antikörper (ABIN115941)

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-CXCL1 Antikörper

Spezifität: Recognises Rat GRO/KC.

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant rGRO/KC.

Anwendungsinformationen

Applikationshinweise: ELISA: To detect rGRO/KC by direct ELISA (using 100 l/well antibody solution) a concentration ofat least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody allowsthe detection of 0.2-0.4 ng/well of recombinant rGRO/KC. Western Blot: To detect rGRO/KC this antibody can be used at a concentration of 0.1-0.2 μg/mL. Thedetection limit for recombinant rGRO/KC is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Neutralisation: To yield one-half maximal inhibition of the biological activity of rat GRO/KC (100 ng/mL) aconcentration of 5-10 μg/mL of this antibody is requirted.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu CXCL1

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Andere Bezeichnung: CXCL1 / GRO-alpha

Hintergrund: The GRO gene was originally identified by subtractive hybridization studies between normal and tumorigenic Chinese hamster embryo fibroblasts. The hamster cDNA was cloned and used as a probe for cloning of the human GRO cDNA. The GROalpha gene initially cloned from T24 cells and the gene in melanoma cells encoding melanoma growth stimulating protein (MGSA) are identical. Human cells contain three closely related, but distinct GRO genes: GRO alpha, GRO beta, and GRO gamma. GRO beta and GRO gamma share 93 % and 82 % identity, respectively, with GRO alpha at the nucleotide level. GROs are members of the chemokine alpha family that is characterized by the separation with one amino acid of the first two cysteine residues, C-X-C, in the amino acid sequence. The GRO gene has been mapped to chromosome 4q21. In normal cells, human mRNA GRO expression is found in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, GRO mRNA has been detected in mammary fibroblasts, mammary epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. Characterization of the GROalpha receptor indicates the presence of low and high affinity receptors on human neutrophils.Synonyms: CXCL1, GRO, GRO-alpha(1-73), GRO1, GROA, Growth-regulated alpha protein, MGSA, Melanoma growth stimulatory activity, NAP-3, Neutrophil-activating protein 3, SCYB1

Gen-ID: 81503

NCBI Accession: NP_110472

UniProt: P14095

Pathways: Autophagie