Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1) Antikörper

Antigen: Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1)

Synonyme: BGP, BGP1, BGPI, 2010110O04Rik, zeh0068, hm:zeh0068, CEACAM8, CEACAM1, MGC142545, Bgp, BGPR, CD66a, Ccam1

Klonalität: Monoklonal (8G5)

Wirt: Maus

Reaktivität: Human, Säugetiere

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Durchflusszytometrie (FACS), Immunfluoreszenz (IF)

Produktnummer: ABIN114594

Produktbeschreibung

Produktmerkmale: Synonyms: BGP, BGP1, Biliary glycoprotein 1, Carcinoembryonic antigen-related cell adhesionmolecule 1

Weitere Bezeichnung: CD66a / CEACAM1

Swiss-Prot: P13688

Immunogen: Genetic immunisation with cDNA encoding the extracellular region of human CEACAM1-A2. Based on recognition of the complete native protein expressed on transfected mammaliancells.

Isotyp: IgG1

Klon: 8G5

Beschreibung: CEACAM1 (BGP/CD66a) is a transmembrane glycoprotein which belongs to thecarcinoembryonic antigen (CEA) gene family (1,2). It is expressed on cells of epithelial andmyeloid origin and mediates homophilic intercellular interactions that influence cellulargrowth, immune cell activation, and tissue morphogenesis. CEACAM1 is a putative tumoursuppressor based on diminished expression in some aggressive types of cancer cells (3). The anti-tumour effect may be due to inhibition of tumour angiogenesis, possibly byincreased secretion of anti-angiogenic molecules from the cells (4). Like all members of theCEACAM family, it consists of a single N domain, with structural homology to theimmunoglobulin variable domains, followed by two immunoglobulin constant-like A (A1,A2) and one B domain. While the N, A1 and B domains can also be found in otherCEA-family members, the A2 domain of CEACAM1 differs from those found in otherCEACAM.

Spezifität: This antibody detects complete native protein expressed on transfected cells. Species: Mammals. Others not tested.

Anwendungen

Applikationshinweise: Flow cytometry: 1.2 μg/10e6 cellsCompetitive ELISA: 1: 200 - 1: 400. ELISA: 1: 200 - 1: 400. Immunoflourescence. Immunohistology: 10 μg/ml (on cryosections). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: PBS, pH 7.2

Lagerung: Store the antibody at 2-8°C for one month or in aliquots at -20°C to -80°C for longer. Werecommend for freezing a shock-freeze procedure using liquid nitrogen. Avoid repeatedfreezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Fig. 1: Specificity testing of GM8G5. BOSC cells were transiently transfected with expression vectors containing the cDNA of either CEACAM1, 3, 4, 5, 6, 7 or 8. Expression of the constructs was tested with monoclonal antibodies known to recognise the corresponding proteins (B, coloured curves, D14HD11, BAC2, 80H3), an irrelevant monoclonal antibody served as negative control (red curves). For specificity testing (A), GM8G5 hybridoma supernatant was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM1 transfected cells (purple curve).

Fig. 2: GM8G5 on renal cell carcinoma (RCC). Hybridoma supernatant of GM8G5 recognises CEACAM1 expressed on the surface of native RCC (orange curve) and on RCC stimulated with IFN- (red curve). PBS served as negative control (black curve).

Fig. 3: Immunohistological staining of colon tissue with GM8G5. Cryosections of colon tissue were stained with 10 ug/ml GM8G5 diluted in PBS containing 2.5% horse serum (B). PBS 2.5% horse serum served as negative control (A). Arrows indicate representa-tive sections of analysed tissues. Detection of GM8G5 occurred with a biotinylated anti-mouse-IgG secondary antibody and a streptavidin-peroxidase conjugate. Diaminobenzidine was used as substrate. Nuclei were stained with hematoxilin