Desmocollin 1 Antikörper

Produktnummer ABIN112238

Der Maus Monoklonal anti-Desmocollin 1 Antikörper wird verwendet zum Nachweis von Desmocollin 1 in Proben von Human. Er wurde validiert für WB, IHC (p) und IHC (fro).

Kurzübersicht für Desmocollin 1 Antikörper (ABIN112238)

Target: Desmocollin 1 (DSC1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmocollin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: Dsc-1-U100

Produktdetails anti-Desmocollin 1 Antikörper

Sequenz: HQTLESVKGV GQGDTGRYAT DWQC

Spezifität: Reacts with 111 kDa (form "a") and 103 kDa (form "b") polypeptides (Desmocollin 1 splice forms, both at pI 5.2) from Human epidermal desmosomes. Mab localizes Dsc1 present in - suprabasal layers of interfollicular epidermis - specific cell layers (e.g. in hair follicle root sheath) - Hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus).

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide corresponding to sequence present within the intracellular part of Human Desmocollin 1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Imunoblotting. Immunohistochemistry on frozen sections: 1: 10, 1 h at RT or overnight at 2-8C,preincubation with 0.05 - 0.2 % Triton X-100, for 5-10 min, depending on tissue type, isrecommended, Without detergent pretreatment a cytoplasmic component mightcross-react (e. g. in fibroblasts). Immunohistochemistry on paraffin-embedded sections (after microwave treatment): 1: 10, 1h at RT or overnight at 2-8C, a slight crossreactivity with a lymphocyte subpopulation isabolished by mild trypsinization after the microwave step)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 1 mL dist. water.

Buffer: PBS buffer, pH 7.4 containing 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Desmocollin 1

Target: Desmocollin 1 (DSC1)

Andere Bezeichnung: Desmocollin-1

Hintergrund: Desmocollin 1 is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It may contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. Desmocollin 1 is linked to the keratinization of epithelial tissues.Synonyms: CDHF1, Cadherin family member 1, DG2/DG3, DSC1, Desmosomal glycoprotein 2/3

Gen-ID: 1823

NCBI Accession: NP_004939

UniProt: Q08554