E-cadherin Antikörper

Produktnummer ABIN111950

Der Maus Monoklonal anti-E-cadherin Antikörper wird verwendet zum Nachweis von E-cadherin in Proben von Human. Er wurde validiert für FACS und IHC (fro).

Kurzübersicht für E-cadherin Antikörper (ABIN111950)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser E-cadherin Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 67A4-2-1

Produktdetails anti-E-cadherin Antikörper

Aufreinigung: Protein A chromatography

Immunogen: Breast carcinoma cell line T47D

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemistry (frozen sections: 4-8 μg/mL, 1/50-1: 100) and FACS. Suggested positive control: Human tonsil. Does not react on routinely processed paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water. For best results, dilute in TBS or other phosphate-free buffer containing 10 mM Ca2+.

Konzentration: 0.4 mg/mL

Buffer: PBS pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.09 % sodium azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Andere Bezeichnung: CD324 / Cadherin-1

Hintergrund: E-Cadherin, also called L-CAM (Liver Cell Adhesion Molecule) is an epithelial cell adhesion molecule and plays an important role in the formation of cell-cell contacts in epithelia irrespective of their origin as ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex and is as such important in maintaining epithelial integrity. Over 90 % of malignancies are carcinomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E-cadherin expression. Therefore, the expression of E-cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm.Synonyms: CAM 120/80, CDH1, CDHE, E-cadherin, Epithelial cadherin, UVO, Uvomorulin

Gen-ID: 999

NCBI Accession: NP_004351

UniProt: P12830

Pathways: WNT Signalweg, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation