F4/80 Antikörper (Biotin)

Produktnummer ABIN111850

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch F4/80 in FACS, WB, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für F4/80 Antikörper (Biotin) (ABIN111850)

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser F4/80 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: Cl:A3-1

Produktdetails anti-F4/80 Antikörper (Biotin)

Spezifität: This antibody recognizes the Murine F4/80 antigen, a 160 kDa cell surface glycoprotein. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (Ref.5).

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of 1/10-1/50 diluted antibody to stain 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Andere Bezeichnung: Macrophage F4/80 Antigen

Hintergrund: F4/80 antigen is a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with human EGF module-containing mucinlike hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480

Gen-ID: 13733

NCBI Accession: NP_034260

UniProt: Q61549