F4/80 Antikörper (APC)

Produktnummer ABIN111848

Der Ratte Monoklonal anti-F4/80 Antikörper wird verwendet zum Nachweis von F4/80 in Proben von Maus. Er wurde validiert für FACS.

Kurzübersicht für F4/80 Antikörper (APC) (ABIN111848)

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser F4/80 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Klon: CI:A3-1

Produktdetails anti-F4/80 Antikörper (APC)

Spezifität: This antibody recognises the Mouse F4/80 antigen, a 160 kDa glycoprotein expressed by murine macrophages. The antibody binds to Macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. The antigen is not expressed by lymphocytes or polymorphonuclear cells.

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Aufreinigung: Affinity Chromatography on Protein A .

Immunogen: Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS-1 mouse myeloma cell line.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL distilled water.

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA 5 % sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!

Details zu F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Andere Bezeichnung: Macrophage F4/80 Antigen

Hintergrund: F4/80 antigen is a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with Human EGF module-containing Mucin-like Hormone Receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480

Gen-ID: 13733

NCBI Accession: NP_034260

UniProt: Q61549