TUBB3 / TUBB4 (Loading Control) Antikörper (FITC)

Antigen: TUBB3 / TUBB4 (Loading Control)

Klonalität: Monoklonal (TU-20)

Wirt: Maus

Konjugat: FITC

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Immunhistochemie (Paraffinschnitte) (IHC (p)), Enzyme Immunoassay (EIA), Durchflusszytometrie (FACS), Western Blot (WB)

Produktnummer: ABIN110981

Produktbeschreibung

Produktmerkmale: Synonyms: Tubulin beta-3 chain, Tubulin beta-III, Tubulin beta-4

Swiss-Prot: Q13509

Immunogen: Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin viacysteine added to the N-terminus of the neuron-specific peptideAA Sequence: ESESQGPK

Isotyp: IgG1

Klon: TU-20

Beschreibung: The betaIII-tubulin isotype is present dominantly in cells of neuronal origin and it is one ofthe earliest marker of neuronal differentiation. Class III beta-tubulin, is regarded as aspecific probe for the cells of neuronal origin as well as for the tumours originating fromthese cells. The neuron-associated class III beta-tubulin isotype is most abundant in cellsof neuronal origin but was also detected in Sertoli cells of the testis and transiently innon-neuronal embryonic tissues.

Spezifität: This antibody recognizes the C-terminal peptide sequence ESESQGPK (aa 441-448) ofhuman class III β-tubulin specific for neurones. MAb TU-20 is widely cross-reactive among species (recognized epitope conserved withinall species).

Anwendungen

Applikationshinweise: Immunohistochemistry on Frozen and Paraffin Sections. Immunocytotochemistry on Fixed and Permeabilized cells. Suggested working dilution is 1/40. The conjugate was also successfully used on FFPE Sections using Confocal Microscopy. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: PBS containing 15 mM Sodium Azide as preservative, approx. pH 7.4Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. Thereagent is free of unconjugated FITC.

Lagerung: Store the antibody in the dark at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar