Interleukin 13 Receptor (IL13R) Antikörper

Antigen: Interleukin 13 Receptor (IL13R)

Klonalität: Monoklonal (GM1E7)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Zell-ELISA (cELISA)

Produktnummer: ABIN108718

Produktbeschreibung

Weitere Bezeichnung: IL-13 receptor

Immunogen: GM1E7 was generated by genetic immunisation of BALB/c mice with an immunisation vector containing the cDNA of the human IL-13Ralpha1 chain extracellular domain (U62858).

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: GM1E7

Beschreibung: Interleukin 13 (IL-13) is a T cell derived cytokine involved in the regulation of inflammatory and immune responses. IL-13Ralpha1 together with IL-4Ralpha forms a functional receptor for both IL-4 and IL-13, which is why these two cytokines share many of their biological activities. The receptor is found on human B cells, monocytes and endothelial cells. However, no functional receptor is expressed on T cells, which explains why IL-13, in contrast to IL-4, fails to induce TH2-cell differentiation. Purified GM1E7 was analysed for potential inhibitory effects on IL-13-induced short-term and long-term cellular responses. To analysise potential antagonistic effects of GM1E7 on IL-13-driven cell proliferation, the IL-13-responsive human cell line TF-1 was employed. Cells were treated with IL-13 in the presence of varied concentrations of purified GM1E7. To prove that GM1E7 specifically inhibits the formation of a signalling competent IL-4R alpha/IL-13R alpha1 heterodimer, but does not interfere with the closely related IL-4R alpha/gamma c complex, a parallel experiment was performed with IL-4 as the mitogen. As shown in Fig. 2, antibody GM1E7 blocks IL-13-dependent cell proliferation completely, but only marginally influences IL-4-activity on TF-1 cells. The IC50 for GM1E7 with regard to IL-13-driven proliferation is in the range of 50 nM.

Spezifität: GM1E7 recognises IL-13Ralpha1 transiently expressed on the cell surface of transfected BOSC cells (Fig. 1) as well as the native protein on monocytes (Myrtek et al., 2004).

Anwendungen

Applikationshinweise: GM1E7 has been tested for the use in flow cytometry and cell-ELISA. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal concent

Lagerung: Store the antibody at 4°C. Avoid repeated freezing and thawing.

Forschungsgebiet: Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Specificity testing of GM1E7. BOSC cells were transiently transfected with an expression vector encoding either the extracellular domain of IL-13Ralpha1 or an irrelevant protein. Expression of the constructs was tested with an anti-tag monoclonal antibody

Specific inhibition of IL-13-dependentproliferation of TF-1 cells by GM-1E7. Samplesof TF-1 cells were incubated for 72 h with 10 nMhuman IL-13 (closed circles) or 10 nM human IL-4(open circles) plus the indicated concentrations ofpurified GM-1E7. Refere

Publikationen

Publikationen: Krause, Behrends, Borowski et al.: "Blockade of interleukin-13-mediated cell activation by a novel inhibitory antibody to human IL-13 receptor alpha1." in: Molecular immunology, Vol. 43, Issue 11, pp. 1799-807, 2006 (PubMed).