Neurofilaments 150, 200 Antikörper

Antigen: Neurofilaments 150, 200

Klonalität: Monoklonal (13AA)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN108435

Produktbeschreibung

Immunogen: Monoclonal antibody to neurofilaments is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Purified neurofilament triplet proteins from human spinal cord were used as immunogen.

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 13AA

Beschreibung: Monoclonal Antibody to Human Cytoskeletal Polypeptides

Spezifität: The antibody reacts with all three neurofilament proteins, Mr 68.000, 150.000 and 200.000 molecules in mouse and recognizes proteins 150.000 and 200.000 in human.

Anwendungen

Applikationshinweise: No reactivity with paraffin sections. Working dilution in western blotting is at least 1:1000

Lagerung: This product should be stored at +2 C to +8 C

Forschungsgebiet: Zytoskelett

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Virtanen I, Miettinen M, Lehto VP et al.: "Diagnostic application of monoclonal antibodies to intermediate filaments." in: Ann N Y Acad Sci, Vol. 455, pp. 635-48, 1985 (PubMed).

Tienari J, Virtanen I, Soinila S et al.: "Neuron-like derivatives of cultured F9 embryonal carcinoma cells express characteristics of parietal endoderm cells." in: Dev Biol, Vol. 123, Issue 2, pp. 566-73, 1987 (PubMed).

Sariola H, Holm K, Henke-Fahle S: "Early innervation of the metanephric kidney." in: Development, Vol. 104, Issue 4, pp. 589-99, 1988 (PubMed).

Karavanov A, Sainio K, Palgi J et al.: "Neurotrophin 3 rescues neuronal precursors from apoptosis and promotes neuronal differentiation in the embryonic metanephric kidney." in: Proc Natl Acad Sci U S A, Vol. 92, Issue 24, pp. 11279-83, 1995 (PubMed).

Szabat E, Rauvala H: "Role of HB-GAM (heparin-binding growth-associated molecule) in proliferation arrest in cells of the developing rat limb and its expression in the differentiating neuromuscular system." in: Dev Biol, Vol. 178, Issue 1, pp. 77-89, 1996 (PubMed).

Murtomaki S, Virtanen I, Liesi P: "Neurofilament proteins are constitutively expressed in F9 teratocarcinoma cells." in: Int J Dev Neurosci, Vol. 17, Issue 8, pp. 829-38, 1999 (PubMed).