Left-Right Determination Factor 2 (LEFTY2) Antikörper

Antigen: Left-Right Determination Factor 2 (LEFTY2)

Synonyme: EBAF, LEFTA, TGFB4, LEFTYA, MGC46222, atv2, cb720, fe38b03, wu:fe38b03, Ebaf, Lefta, Leftb, AV214969, MGC98569, 6030463A22Rik, Ebaf2, LEFTY2, LEFTY1

Klonalität: Monoklonal (7C5G1H6H10)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus), Schaf

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN103459

Produktbeschreibung

Weitere Bezeichnung: LEFTY A

Immunogen: A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells using conventional hybridoma technology.

Format: Liquid (sterile filtered)

Isotyp: IgG2a kappa  (Passende Sekundärantikörper)

Klon: 7C5G1H6H10

Beschreibung: During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.

Spezifität: This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY. No reactivity occurs against 6X HIS tag. Significant sequence homology exists between the human and mouse forms of LEFTY. Cross reactivity is expected with mouse and rat forms of the protein. Reactivity with other sources has not been determined.

Anwendungen

Applikationshinweise: Recommended Dilutions: For ELISA dilute the antibody 1:5,000. For western blotting dilute the antibody 1:2,000. Researchers should determine optimal titers for other applications. Note: This antibody is suitable for ELISA and western blotting. The antibody may be used for other applications, such as RIA, immunohistochemistry or immunoprecipitation, but specific reaction conditions have not been developed.

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Mab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. Detection occurs after 1.0 ug of protein is loaded in each lane. The blot was incubated with a 1:2,000 dilution of Mab anti-Human LEFTY at room temperature for 30 min followed by detection using IRDye