Kaninchen anti-Maus IgG (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed

Produktnummer ABIN101786

Hersteller Produkt- Nr.: 610-4520

Kurzübersicht für Kaninchen anti-Maus IgG (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN101786)

Target: IgG

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Maus

Wirt: Kaninchen

Hersteller Produkt- Nr.: 610-4520

Hersteller: Rockland

Verwendungszweck: Mouse IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgG and Mouse Serum.  No reactivity was observed against Human serum proteins.

Produktmerkmale: Anti-Mouse IgG ATTO425 Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose followed by solid phase adsorption to remove unwanted reactivities and extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen: Optional[Immunogen]: Mouse IgG whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Mouse IgG Alkaline Phosphatase conjugate has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:100 - 1:500 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:9,800 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.