HER3 Antikörper

Antigen: HER3

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus, Human, Ratte (Rattus)

Applikation: Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN100396

Produktbeschreibung

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a GST-HER3 fusion protein corresponding to amino acids 1283 to 1323 (40 amino acids) at the carboxy-terminus of human HER3.

Format: Liquid (sterile filtered)

Beschreibung: The human Epidermal Growth Factor Receptor (EGFR) family is a family of receptor tyrosine kinases consisting of 4 members: EGFR, HER2, HER3 and HER4. These receptors are expressed on a wide variety of tissue types and have been implicated in many human cancers, including breast, gastric, colon and prostate. Strong expression of both HER3 and HER2 (also known as p185neu) by tumor cells can be associated with poor outcome for patients. The ligand for HER3 and HER4 is called Neu Differentiation Factor (NDF) or Heregulin. In some instances, antibodies against these receptors prove effective in combination therapy regimens for treatment of the disease. Signaling through the EGFR family involves the combinatorial dimerization of the four known family members and is mediated by at least 8 different hormones, including EGF and the heregulins. Heregulin binding and tyrosine phosphorylation by HER3 or HER4 is enhanced in the presence of HER2. Structurally the members of the EGFR family are 1210 to 1342 amino acids in length with approximately 650 amino acids of the extracellular domain being heavily glycosylated. The extracellular domains show up to 70% sequence homology.

Spezifität: This product is an IgG fraction antibody purified from monospecific antiserum by Protein A chromatography followed by cross adsorption against GST and extensive dialysis against the buffer stated above. Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Rabbit Serum. The antibody is directed against human HER3 and is useful in determining its presence in immunoprecipitation experiments. This antibody can detect HER3 from human, mouse and rat sources. Reactivity of this antibody with HER3 from other species is unknown.

Anwendungen

Applikationshinweise: Note: Anti-HER3 antibody is specifically designed for immunoprecipitation and can be used for immunoblotting in conjunction with a detection antibody like anti-phosphotyrosine. Reactivity in other assays is likely, but has not been determined. Recognition of HER3 is independent of the phosphorylation status of the molecule. Human prostate cell lines (LNCap) or human breast cell line (MDA-453) are typically used as positive control sources. Combined immunoprecipitation and immunoblot of a human cell lysate using anti-HER3 antibody. Lysates were prepared from a human LNCap prostate cell line both with (+) treatment with Heregulin- beta1 for 15' at 100 ng/ml and without (-) the addition of Heregulin- beta1. The combination of immunoprecipitation and immunoblotting was performing using 5 ul of the anti-HER3 antibody for immunoprecipitation followed by immunoblot detection using an anti-phosphotyrosine antibody. Visualization occurred using an ECL system. Film exposure approximately 1. Other detection systems will yield similar results. IP anti-HER3 (5 ul) IB anti-pTyr - + 180 kDa.

Konzentration: 1.0 mg mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. For extended storage aliquot contents and freeze at or below -20° C. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Zytokine, Rezeptoren

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Combined immunoprecipitation and immunoblot of a human cell lysate using anti-HER3 antibody. Lysates were prepared from a human LNCap prostate cell line both with (+) treatment with Heregulin-beta1 for 15' at 100 ng/ml and without (-) the addition of Heregulin-beta1. The combination of immunoprecipitation and immunoblotting was performing using 5 uL of the anti-HER3 antibody for immunoprecipitation followed by immunoblot detection using an anti-phosphotyrosine antibody. Visualization occurred using an ECL system. Film exposure approximately 1'. Other detection systems will yield similar results.