beta Defensin 1 Protein

Produktnummer ABIN987768

Produktdetails

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Sequenz: GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCC

Produktmerkmale: Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay usingCD34+ dendritic cells is less than 1000 ng/ml, corresponding to a specific activity of >, 1.0 × 103 IU/mg.

Reinheit: > 98 % by SDS-PAGE and HPLC analyses.

Endotoxin-Niveau: Level Less than 1EU/µg of rHuBD-1 as determined by LAL method

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Lagerung: 4 °C

Antigendetails

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Andere Bezeichnung: beta-Defensin 1 (BD-1) (DEFB1 Produkte)

Hintergrund: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The ª-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified, BD-1, BD-2, BD-3 and BD-4. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. Synonym: beta-Defensin 1 (BD-1), Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 130mM NaCl.

Molekulargewicht: Approximately 5.0 KDa, a single non-glycosylated polypeptide chain containing 47 amino acids.