custom-made
MT-CYB
Spezies: Maus
Wirt: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cytochrome b (MT-CYB)
(Mitochondrially Encoded Cytochrome B (MT-CYB))
Protein-Typ
Recombinant
Proteineigenschaft
AA 1-381
Spezies
Maus
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses Cytochrome b Protein ist gelabelt mit His tag.
Verwendungszweck
Made-to-order recombinant Mt-Cyb Protein expressed in mammalian cells.
Sequenz
MTNMRKTHPL FKIINHSFID LPAPSNISSW WNFGSLLGVC LMVQIITGLF LAMHYTSDTM TAFSSVTHIC RDVNYGWLIR YMHANGASMF FICLFLHVGR GLYYGSYTFM ETWNIGVLLL FAVMATAFMG YVLPWGQMSF WGATVITNLL SAIPYIGTTL VEWIWGGFSV DKATLTRFFA FHFILPFIIA ALAIVHLLFL HETGSNNPTG LNSDADKIPF HPYYTIKDIL GILIMFLILM TLVLFFPDML GDPDNYMPAN PLNTPPHIKP EWYFLFAYAI LRSIPNKLGG VLALILSILI LALMPFLHTS KQRSLMFRPI TQILYWILVA NLLILTWIGG QPVEHPFIII GQLASISYFS IILILMPISG IIEDKMLKLY P Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
Spezifität
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
Produktmerkmale
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Reinheit
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
MT-CYB
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
MT-CYB
Spezies: Human
Wirt: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
SDS, ELISA, WB
custom-made
MT-CYB
Spezies: Maus
Wirt: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
SDS, ELISA, WB
MT-CYB
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
> 90 %
SDS
Applikationshinweise
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
The buffer composition is at the discretion of the manufacturer.
Handhabung
Avoid repeated freeze-thaw cycles.
Lagerung
-80 °C
Informationen zur Lagerung
Store at -80°C.
Haltbarkeit
12 months
Target
Cytochrome b (MT-CYB)
(Mitochondrially Encoded Cytochrome B (MT-CYB))
Cytochrome b (Complex III subunit 3) (Complex III subunit III) (Cytochrome b-c1 complex subunit 3) (Ubiquinol-cytochrome-c reductase complex cytochrome b subunit),FUNCTION: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis. {ECO:0000250|UniProtKB:P00157}.